Figure 6

Intracellular concentration of metabolites under aerobic conditions (P-0) and after anaerobic residence time (P-5) in two-compartment cultivation TCR2. Quantified by metabolic quenching/LC-MS/MS [27] in batch and fed-batch phase of C. glutamicum cultivation with oscillation of τ = 87 s (TCR2); (A) = adenosine phosphates (left axis) and resulting energy charge (right axis, calculation and definition of energy charge see Methods); (B) = redox cofactor equivalents, batch = substrate excess and oscillation of oxygen supply, feed = substrate limitation and oscillation of both substrate and oxygen supply; AMP = adenosine monophosphate, ADP = adenosine diphosphate, ATP = adenosine triphosphate, NAD = nicotinamide adenine dinucleotide (oxidized), NADH = reduced, NADP = nicotinamide adenine dinucleotide phosphate (oxidized), NADPH = reduced.