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Table 5 Composition of the HSG medium

From: Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)

Medium component

Medium concentration/g L−1

Soy peptone

13.5

Yeast extract

7

Glycerol

14.9

NaCl

2.5

K2HPO4

2.3

KH2PO4

1.5

MgSO4· H20

0.14