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Table 4 Composition of the SGA medium

From: Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)

Solution name

Volume fraction/%

Component

Solution concentration/g L−1

Medium concentration/g L−1

Micronutrient solution 500×

0.2

FeCl3· 6H20

5.4

0.0108

ZnSO4· 7H2O

1.38

0.00276

MnSO4· H20

1.85

0.0037

CoSO4· 7H20

0.56

0.00112

CuCl2

0.17

0.00034

H3BO3

1

0.002

Na2MoO4· 2H2O

2.5

0.005

Citric acid hydrate

5

0.01

Salt solution 10×

10

K2HPO4

135.3

13.53

KH2PO4

66.2

6.62

Citric acid hydrate

18.4

1.84

EDTA

0.08

0.008

Glycerol solution

1

Glycerol

1,000

10

Glucose solution

10

Glucose

195.6

19.56

Ammonium sulphate solution

1.2

(NH4)2SO4

500

6

MgSO4 solution

0.24

MgSO4

250

0.6