Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)

Table 3 List of plasmids used

Plasmid	Features/Genes	Source
p582	rep (pUC19), bla (Amp^R), npt (Kan^R), P_CP7-bgl, P_fic-kil, T7 terminator, multiple cloning site	[21]
pJET 1.2	rep (pMB1), bla (Amp^R), eco47IR, P_lacUV5, T7 promoter, multiple cloning site, insertion site	Fermentas
pJET-tpiA	rep (pMB1), bla (Amp^R), eco47IR, P_lacUV5, T7 promoter, residual multiple cloning site, tpiA expression cassette	This work
pFC1	rep (pUC19), bla (Amp^R), npt (Kan^R), P_CP7-bgl, P_fic-kil, T7 terminator, tpiA expression cassette in forward orientation	This work
pFC4	rep (pUC19), bla (Amp^R), npt (Kan^R), P_CP7-bgl, P_fic-kil, T7 terminator, tpiA expression cassette in reverse orientation	This work
pJET-tpiA-Tart	rep (pMB1), bla (Amp^R), eco47IR, T7 promoter, residual multiple cloning site, tpiA expression cassette, artificial terminator	This work

ISSN: 1475-2859