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Table 1 Primers for cloning an artificial terminator behind the tpiA gene on pJET-tpiA

From: Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)

Primer name

Sequence

tpiA Term. lacUV

5′-CGCCCCTTTTTTTTTagcctggggtgcctaatgag-3′

tpiA Term. direct

5′-GCTTCGCCCCTTTTTTTTTTTaagcctgtttagccgcttctgc-3′