From: Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)
Primer name
Sequence
tpiA Term. lacUV
5′-CGCCCCTTTTTTTTTagcctggggtgcctaatgag-3′
tpiA Term. direct
5′-GCTTCGCCCCTTTTTTTTTTTaagcctgtttagccgcttctgc-3′