Figure 8From: Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA) Growth of the parent strain for the Keio Collection BW25113 and JW3890-2 deletion mutant strains. The mutant strains were grown in the absence or presence of the complementing plasmid pJET-tpiA-Tart. The cultivations proceeded in shake flasks on the glucose-based HSG medium.Back to article page