Figure 4From: Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA) Batch cultivation of E. coli strain JW3890-2-pFC1. The Keio ΔtpiA knockout strain JW3890-2 complemented with the construct pFC1 was grown in SGA medium in a 2 L in-house fermenter with 1 L working volume at 30°C without the use of antibiotics.Back to article page