From: Expression of endoglucanases in Pichia pastoris under control of the GAP promoter
Run | Strain | Temperature (°C) | Feed | Feed regime | Max dry cell weight (g/l) | Total supernatant protein (g/l) |
---|---|---|---|---|---|---|
1 | GAP/TaCel5A | 30 | Glycerol | 1714 g over 70 h | 109 | 2.8 |
2b | GAP/TaCel5A | 30 | Glycerol | 2470 g over 70 h | 155 ± 5 | 3.8 ± 0.4 |
3 | GAP/TaCel5A | 30 | Glucose | 2470 g over 70 h | 145 | 4.9 |
4 | GAP/TaCel5A | 25 | Glycerol | 2470 g over 70 h | 155 | 5.4 |
5 | GAP/AfCel12A | 30 | Glycerol | 1714 g over 70 h | 132 | 3.4 |
6b | GAP/AfCel12A | 30 | Glycerol | 2470 g over 70 h | 146 ± 9 | 3.3 ± 0.3 |
7 | GAP/AfCel12A | 30 | Glucose | 2470 g over 70 h | 100 | n.d.a |
8 | GAP/AfCel12A | 25 | Glycerol | 2470 g over 70 h | 170 | 7.3 |