Figure 2
From: Expression of endoglucanases in Pichia pastoris under control of the GAP promoter
Endoglucanase production in 3-liter bioreactors with fed-batch cultivation. One clone of each PichiaPink™ recombinant strain was grown in two parallel 3-l fermenters. The SDS-PAGE figures show the accumulation of A) Af Cel12A and B) Ta Cel5A. Sample lanes are marked with the age of the culture in hours for each fermenter. Reactor B of AOX1/AfCel12A is missing from the gel as cells stopped growing in the batch phase. The bands appearing at approx. 24 kDa (panel A) represent Af Cel12A; the bands appearing at approx. 37 kDa represent Ta Cel5A (panel B).