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Table 4 Strains and plasmids used

From: Enhancement of acetoin production in Candida glabrata by in silico-aided metabolic engineering

Strains/plasmids

Relevant characteristics

Reference

Plasmids

  

pY26

2 μm, AmpR, URA3, P GPD , P TEF

Lab. collection

pYX212

2 μm, AmpR, URA3, P TPI

Lab. collection

pY36

2 μm, AmpR, URA3, P TEF , P TEF

This study

pY16

2 μm, AmpR, URA3, P TPI , P TPI

This study

pY46

2 μm, AmpR, URA3, P GPD , P GPD

This study

pYX212-BaALS

Codon-optimized ALS from B. amyloliquefaciens

This study

pYX212-BpALS

Codon-optimized ALS from Bacillus pumilus

This study

pYX212-BsALS

Codon-optimized ALS from B. subtilis

This study

pYX212-EcALS

Codon-optimized ALS from E. coli

This study

pYX212-BaALDC

Codon-optimized ALDC from B. amyloliquefaciens

This study

pYX212-BpALDC

Codon-optimized ALDC from B. pumilus

This study

pYX212-BsALDC

Codon-optimized ALDC from B. subtilis

This study

pYX212-EcALDC

Codon-optimized ALDC from E. coli

This study

pY16-TPI-BsALS-TPI-BaALDC

Codon-optimized ALS and ALDC under TPI promoter

This study

pY26-TEF-BsALS-GPD-BaALDC

Codon-optimized ALS and ALDC under TEF and GPD promoter

This study

pY26-GPD-BsALS-TEF-BaALDC

Codon-optimized ALS and ALDC under GPD and TEF promoter

This study

pY36-TEF-BsALS-TEF-BaALDC

Codon-optimized ALS and ALDC under TEF promoter

This study

pY46-GPD-BsALS-GPD-BaALDC

Codon-optimized ALS and ALDC under GPD promoter

This study

strains

  

C. glabrata CCTCC M202019

Multivitamin (thiamine, biotin, nicotinic acid and pyridoxine) auxotroph

[37]

C. glabrata Δura 3ΔArg8

The mutant derived from C. glabrata CCTCC M202019

[36]

MuA0

C. glabrata Δura3 ΔArg8 (pYX212)

This study

MuA1

C. glabrata Δura3 ΔArg8 (pYX212-BaALS)

This study

MuA2

C. glabrata Δura3 ΔArg8 (pYX212-BpALS)

This study

MuA3

C. glabrata Δura3 ΔArg8 (pYX212-BsALS)

This study

MuA4

C. glabrata Δura3 ΔArg8 (pYX212-EcALS)

This study

MuA5

C. glabrata Δura3 ΔArg8 (pYX212-BaALDC)

This study

MuA6

C. glabrata Δura3 ΔArg8 (pYX212-BpALDC)

This study

MuA7

C. glabrata Δura3 ΔArg8 (pYX212-BsALDC)

This study

MuA8

C. glabrata Δura3 ΔArg8 (pYX212-EcALDC)

This study

MuA9

C. glabrata Δura3 ΔArg8 (pY16-TPI-BsALS-TPI-BaALDC)

This study

MuA10

C. glabrata Δura3 ΔArg8 (pY46-GPD-BsALS-GPD-BaALDC)

This study

MuA11

C. glabrata Δura3 ΔArg8 (pY36-TEF-BsALS-TEF-BaALDC)

This study

MuA12

C. glabrata Δura3 ΔArg8 (pY26-GPD-BsALS-TEF-BaALDC)

This study

MuA13

C. glabrata Δura3 ΔArg8 (pY26-TEF-BsALS-GPD-BaALDC)

This study

MuA14

C. glabrata Δura3 ΔArg8 Δilv5 (pY26-TEF-BsALS-GPD-BaALDC)

This study

MuA15

C. glabrata Δura3 ΔArg8 ΔADH (pY26-TEF-BsALS-GPD-BaALDC)

This study