From: Enhancement of acetoin production in Candida glabrata by in silico-aided metabolic engineering
Parameters* | Strains | ||
---|---|---|---|
MuA13 | MuA14 | MuA15 | |
DCW (g/L) | 10.27 ± 0.5 | 9.96 ± 0.4 | 10.13 ± 0.5 |
Pyruvate (g/L) | 18.4 ± 0.7 | 16.8 ± 0.8 | 16.3 ± 1.1 |
α-acetolactate | 0.72 ± 0.02 | 0.81 ± 0.01 | 0.74 ± 0.02 |
Specific activity of ALS (U/mg protein) | 1.05 ± 0.03 | 1.07 ± 0.05 | 1.04 ± 0.04 |
Specific activity of ALDC (U/mg protein) | 2.06 ± 0.06 | 2.03 ± 0.05 | 2.10 ± 0.1 |
Acetoin (g/L) | 1.96 ± 0.3 | 2.13 ± 0.3 | 2.08 ± 0.2 |
Acetoin yield (g/g DCW) | 0.19 | 0.21 | 0.21 |
2,3-Butanediol (g/L) | 1.36 ± 0.03 | 1.45 ± 0.07 | 2.03 ± 0.03 |
Acetoin/2,3-Butanediol | 1.44 | 1.47 | 1.02 |
Ethanol (g/L) | 3.67 ± 0.11 | 2.96 ± 0.13 | 2.72 ± 0.2 |
Acetate (g/L) | 0.64 ± 0.05 | 0.51 ± 0.05 | 0.83 ± 0.1 |
Glycerol (g/L) | 1.01 ± 0.1 | 1.07 ± 0.12 | 1.12 ± 0.12 |
Intracellular NAD+ (mg/g DCW) | 16.1 ± 1.2 | 14.6 ± 1.7 | 15.8 ± 2.1 |
Intracellular NADH/NAD+ ratio | 0.62 | 0.61 | 0.64 |