Table 2 The comparison of fermentation characteristics of the engineered strains MuA0 and MuA9
From: Enhancement of acetoin production in Candida glabrata by in silico-aided metabolic engineering
Parametersa | Strains | Fold change, B/A-1 | |
|---|---|---|---|
MuA0 (A) | MuA9 (B) | ||
DCW (g/L) | 10.84 ± 0.6 | 10.34 ± 0.5 | -0.05 |
Pyruvate (g/L) | 30.7 ± 1.2 | 24.4 ± 0.8 | -0.20 |
Specific activity of ALS (U/mg protein) | 0.16 ± 0.01 | 0.83 ± 0.04 | 4.19 |
Specific activity of ALDC (U/mg protein) | ND | 1.86 ± 0.03 | -- |
Acetolactate (g/L) | 0.12 ± 0.01 | 0.74 ± 0.01 | 5 |
Acetoin (g/L) | 0.042 ± 0.008 | 1.14 ± 0.05 | 26 |
Acetoin yield (g/g DCW) | 0.003 | 0.11 | 35 |
2,3-Butanediol (g/L) | 0.035 ± 0.004 | 0.88 ± 0.04 | 24 |
Diacetyl (g/L) | 0.01 ± 0.00 | ND | -- |
Ethanol (g/L) | 0.87 ± 0.02 | 2.15 ± 0.2 | 1.47 |
Glycerol (g/L) | 0.66 ± 0.04 | 1.15 ± 0.1 | 0.74 |