Enhancement of acetoin production in Candida glabrata by in silico-aided metabolic engineering

Table 1 Effects of gene over-expression on the enzymes and metabolites ^a

Strains	Gene	Species	ALS	α-acetolactate	Diacetyl	ALDC	Acetoin
			(U/mg protein)	(g/L)	(g/L)	(U/mg protein)	(g/L)
MuA0	nt	nt	0.15 ± 0.01	0.11 ± 0.01	0.02 ± 0.00	ND	0.041 ± 0.002
MuA1	alsS	B. amyloliquefaciens	0.75 ± 0.05	0.58 ± 0.02	0.37 ± 0.02	ND	0.054 ± 0.003
MuA2	alsS	B. pumilus	0.60 ± 0.05	0.47 ± 0.03	0.33 ± 0.03	ND	0.053 ± 0.001
MuA3	alsS	B. subtilis	0.81 ± 0.05	0.70 ± 0.05	0.45 ± 0.02	ND	0.077 ± 0.004
MuA4	alsS	E. coli	0.77 ± 0.1	0.62 ± 0.04	0.42 ± 0.04	ND	0.065 ± 0.002
MuA5	alsD	B. amyloliquefaciens	0.15 ± 0.01	0.11 ± 0.01	ND	1.85 ± 0.05	0.14 ± 0.04
MuA6	alsD	B. pumilus	0.14 ± 0.012	0.11 ± 0.01	ND	1.66 ± 0.05	0.12 ± 0.03
MuA7	alsD	B. subtilis	0.15 ± 0.02	0.12 ± 0.02	ND	1.71 ± 0.05	0.13 ± 0.06
MuA8	alsD	E. coli	0.13 ± 0.01	0.10 ± 0.02	ND	1.57 ± 0.1	0.12 ± 0.02

^aThe engineered strains were grown in fermentation medium, and the production of metabolites in the culture supernatant was determined by HPLC at 64 h during batch flask culture. Results are the average of three replicates with error bars indicating standard error from the mean. ND, Not detected; nt, Not.

ISSN: 1475-2859