Table 2 Optical signals and applied setup for BioLector on-line monitoring
From: Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems
Optical signal | λex[nm] | λem[nm] | Gain |
|---|---|---|---|
Biomass (scattered light) | 620 | - | 20 |
DOT | 505 | 590 | 60 |
pH | 485 | 530 | 45 |
YFP fluorescence | 510 | 532 | 60 |
FbFP fluorescence | 450 | 492 | 60 |
mCherry fluorescence | 580 | 610 | 60 |
GFP fluorescence | 488 | 520 | 100 |