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Figure 5 | Microbial Cell Factories

Figure 5

From: Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems

Figure 5

Cultivation of K. lactis GG799 expressing recombinant GFP using the BioLector system. Online monitoring of microbial growth (via scattered light) and DOT (A), pH value and fluorescence intensity of the recombinant expressed GFP. Additional pH measurement of offline samples (B). Cultivation conditions: 48well FlowerPlate with optodes for DOT and pH online measurement, VL = 800 μL, n = 1100 rpm, d0 = 3 mm, 30°C, YNB medium with 20 g/L galactose as substrate and inducer.

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