Figure 2
From: Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems
Absorbance spectra of purified fluorescent proteins (A) and 2D fluorescence spectra of E. coli cell suspensions after the expression of the fluorescent proteins YFP (B), FbFP (C) and mCherry (D). Measuring points for optical monitoring of microbial growth (via scattered light), DOT and pH indicated by dotted lines (B-D). Cultivation conditions: 250 mL shaking flask, VL = 10 mL, n = 350 rpm, d0 = 50 mm, 37°C, 24 h, Wilms-MOPS medium with 20 g/L glucose, induction with 0.1 mM IPTG after 6 h. λex,pH and λex,DOT indicate for excitation wavelengths for optical pH and DOT monitoring. Note: Altered color code for Figure 2B (upper scale) and Figures 2C and D (lower scale).