Skip to main content

Table 1 Yield, endotoxin level, and purity of recombinant MAP in each purification step

From: Highly purified mussel adhesive protein to secure biosafety for in vivo applications

Step

Whole cells

Inclusion body isolation after disruption of CaCl2/EDTA-treated cells

Acetic acid extraction after Triton X-114 double washing of inclusion body

Cation exchange chromatography of extracted fraction

Total weight (mg)

25,500.00

9,600.00

516.00

275.47

Production yield (μg/mL)

7,697.80

7,309.75

2,956.25

1,578.21

Relative production yield (%)

100.00

94.96

38.40

20.50

Endotoxin level (EU/mg-total protein)

108,332.01

6,324.79

403.45

22.33

Relative endotoxin level (%)

100.00

5.84

0.37

0.02

Purity (%)

-

35.30a)

89.81b)

99.90b)

  1. a)calculation based on SDS-PAGE analysis.
  2. b)calculation based on HPLC analysis.