From: Highly purified mussel adhesive protein to secure biosafety for in vivo applications
Step | Whole cells | Inclusion body isolation after disruption of CaCl2/EDTA-treated cells | Acetic acid extraction after Triton X-114 double washing of inclusion body | Cation exchange chromatography of extracted fraction |
---|---|---|---|---|
Total weight (mg) | 25,500.00 | 9,600.00 | 516.00 | 275.47 |
Production yield (μg/mL) | 7,697.80 | 7,309.75 | 2,956.25 | 1,578.21 |
Relative production yield (%) | 100.00 | 94.96 | 38.40 | 20.50 |
Endotoxin level (EU/mg-total protein) | 108,332.01 | 6,324.79 | 403.45 | 22.33 |
Relative endotoxin level (%) | 100.00 | 5.84 | 0.37 | 0.02 |
Purity (%) | - | 35.30a) | 89.81b) | 99.90b) |