Figure 3From: Highly purified mussel adhesive protein to secure biosafety for in vivo applications (A) Ion exchange chromatogram, (B) SDS-PAGE analysis, and (C) endotoxin level determination of recombinant MAP sample after ion exchange chromatography purification. The data represent mean +/− standard deviation and were analyzed 2-tails student t-test (*p < 0.05, **p < 0.01, ***p < 0.005). Abbreviations: MW, molecular weight marker; WC, whole cells; X-114/114, acetic acid-extracted MAP after inclusion body washing with Triton X-114 twice; P1, first peak fraction from cation exchanger of acetic acid-extracted solution after inclusion body washing with Triton X-114 twice; P2, second peak fraction from cation exchanger of acetic acid-extracted solution after inclusion body washing with Triton X-114 twice; ppMAP, purified MAP based on previous method.Back to article page