Figure 2
From: Highly purified mussel adhesive protein to secure biosafety for in vivo applications
(A) SDS-PAGE analysis and (B) endotoxin level determination of recombinant MAP samples according to each purification step before ion exchange chromatography. The data represent mean +/− standard deviation and were analyzed 2-tails student t-test (*p < 0.05, **p < 0.01, ***p < 0.005). Abbreviations: MW, molecular weight marker; WC, whole cells; IB, isolated inclusion body containing MAP after disruption of CaCl2/EDTA-treated whole cells; X-100/100, acetic acid-extracted MAP after inclusion body washing with Triton X-100 twice; X-100/114, acetic acid-extracted MAP after inclusion body washing with Triton X-100 and Triton X-114 in order; X-114/100, acetic acid-extracted MAP after inclusion body washing with Triton X-114 and Triton X-100 in order; X-114/114, acetic acid-extracted MAP after inclusion body washing with Triton X-114 twice.