Table 1 Comparison of specific fluorescence of GFP expressed under control of lac (pAB828) and soxS (pAB49) promoters
From: Production of recombinant protein by a novel oxygen-induced system in Escherichia coli
Parameter (units) | pAB828 batch, 30% dO2 | pAB828 batch, 300% dO2 | pAB49 batch, 300% dO2 | pAB49 fed-batch, 300% dO2 |
|---|---|---|---|---|
Amount of GFP in the total soluble protein (%) | 5.1 ± 0.31 | 4.9 ± 0.38 | 1.5 ± 0.03 | 3.4 ± 0.11 |
Specific fluorescence (FU/μg GFP) | 13753 ± 880 | 14464 ± 1065 | 10831 ± 191 | 11617 ± 1048 |