Table 2 Oligonucleotides used in this study
From: Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer
Oligonucleotide | Sequence |
|---|---|
P-pVS13_for_long | CAGCGTGTAGACAAACCTTCGCATTC |
P-pVS13_for_short | CCTTCGCATTCGTTGTCAGGTCTGC |
P-pVS13/14_rev_long | TCCACATCCCTTTTTTCTTATTTCAGAATAATCATC |
P-pVS13/14_rev_short | TCTTATTTCAGAATAATCATCCGTAGTCTATAAGAATG |
P- pVS13/14_RG_for_long | AAAAAGGGATGTGGAATGATGAGGAAAAAGAGTTTTTG |
P- pVS13/14_RG_for_short | ATGATGAGGAAAAAGAGTTTTTGGCTTGGGATGC |
P- pVS13/14_RG_rev_short | TTGCTCAAAAAAATCTCGGTCAGATGTTACTAGCAACTC |
P- pVS13_RG_rev_long | TTTGTCTACACGCTGTTGCTCAAAAAAATCTCGGTCAG |
P- pVS14_for_long | ATGACAAAAACAATGATAAAATAATATTTTTTTATATCG |
P- pVS14_for_short | ATAAAATAATATTTTTTTATATCGAAATTCGAAATAGCTGC |
P- pVS14_RG_rev_long | CATTGTTTTTGTCATTTGCTCAAAAAAATCTCGGTCAG |
P-pVS19_for_long | AGCAACTGGATCTAACAAGAGGAAAGGCCGCC |
P- pVS19_for_short | TAACAAGAGGAAAGGCCGCCAATTAG |
P- pVS19_rev_long | TTTTCCTCATCATCATATTCCTCCTTTATGTCCTATATCAAAAATC |
P- pVS19_rev_short | CATATTCCTCCTTTATGTCCTATATCAAAAATCATACG |
P-pVS18_RG_for_long | ATGATGAGGAAAAAGAGTTTTTGGCTTG |
P-pVS18_RG_for_short | AGAGTTTTTGGCTTGGGATGCTGAC |
P-pVS18_RG_rev_long | GATCCAGTTGCTCAAAAAAATCTCGGT |
P-pVS18_RG_rev_short | CAAAAAAATCTCGGTCAGATGTTACTAGCA |
P-pVS20_for_long | AGCAACTGGATCCGAGAACATCTTGAAAGGCA |
P-pVS20_for_short | CGAGAACATCTTGAAAGGCAGCACAGC |
P-pVS20_rev_long | TTTTCCTCATCATAATACCCACTCTCCCTTTCATCTTTTTGTC |
P-pVS20_rev_short | AATACCCACTCTCCCTTTCATCTTTTTGTC |
P-pVS23_for | GAGCAATTTTAAATGACAAAAACAATGATAAAATAATATTTTTT |
P-pVS23_for_short | ACAAAAACAATGATAAAATAATATTTTTTTATATCGAAATTCGAAATAG |
P-pVS23_rev | CATTTAAAATTGCTCAAAAAAATCTCGGTCAGATG |
P-pVS23_rev_short | AAAAAAATCTCGGTCAGATGTTACTAGCAACTCA |
P-pVS24_for | CTGTTATATAAACAGGTTCTTTTAAATGACAAAAACAATG |
P-pVS24_for_short | AGGTTCTTTTAAATGACAAAAACAATGATAAAATAATATTTTTTTATATCG |
P-pVS24_rev | GTTTATATAACAGGTTCTTGCTCAAAAAAATCTCGGTCAGATG |
P-pVS24_rev_short | GTTCTTGCTCAAAAAAATCTCGGTCAGATG |
pHP5-31_backbone_for_long | ATGACGATGAGAAAACGTAAAAATGG |
pHP5-31_backbone_for_short | GTAAAAATGGATTAATCAGTATTCTATTGGC |
pHP5-31_backbone_rev_long | CATTTACAAGAACAGCATCTTTCCTCG |
pHP5-31_backbone_rev_short | CATCTTTCCTCGTTTTTCTTGTACCTG |
pTK5/6/7_Insert_for_long | CTGTTCTTGTAAATGAGTTGCTAGTAACATCTG |
pTK5/6/7_Insert_for_short | AGTTGCTAGTAACATCTGACCGAGATTTTTTTGAGC |
pTK5_Insert_rev_long | GTTTTCTCATCGTCATCATATTCCTCCTTTATGTC |
pTK5_Insert_rev_short | CATATTCCTCCTTTATGTCCTATATCAAAAATC |
pTK6_Insert_rev_long | GTTTTCTCATCGTCATTCCACATCCCTTTTTTC |
pTK6_Insert_rev_short | TCCACATCCCTTTTTTCTTATTTCAGAATAATC |
pTK7_Insert_rev_long | GTTTTCTCATCGTCATAATACCCACTCTCCCTTTCATC |
pTK7_Insert_rev_short | AATACCCACTCTCCCTTTCATCTTTTTGTC |