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Table 1 Bacterial strains and plasmids used in this contribution

From: Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer

Strain/Plasmid Description Reference
Strains   
B. subtilis DB104 his nprR 2 nprE 18 ΔaprA 3 Kawamura & Doi [26]
B. pumilus Jo2 Wild type Henkel AG & Co. KGaA
B. pumilus Jo2.1 Jo2 Δyqf D Wemhoff et al. [13]
Plasmids   
pHP49 pBC16, Pref, subtilisin BL18, KmR Henkel AG & Co. KGaA
pHP59 pBC16*, Pref, subtilisin BL18, KmR Henkel AG & Co. KGaA
pPB49 pUB110, Pref, subtilisin BL18, KmR Henkel AG & Co. KGaA
pHP17 pUB110*, Pref, subtilisin BL18, KmR Henkel AG & Co. KGaA
pVS13 pHP49, but P apr E1 I This work
pVS24 pHP49, but P apr E1 II This work
pVS23 pHP49, but P apr E1 III This work
pVS14 pHP49, but P apr E1 IV This work
pVS19 pHP49, but P mpr This work
pVS20 pHP49, but P apr E2 This work
pHP5-31 pBC16, Pref, α-amylase, KmR Henkel AG & Co. KGaA
pTK5 pHP5-31, but P mpr This work
pTK6 pHP5-31, but P apr E1 III This work
pTK7 pHP5-31, but P apr E2 This work
  1. See Additional file 1 for cloning details (*non-relevant backbone regions deleted).