Table 1 Bacterial strains and plasmids used in this contribution
From: Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer
Strain/Plasmid | Description | Reference |
|---|---|---|
Strains | ||
B. subtilis DB104 | his nprR 2 nprE 18 ΔaprA 3 | Kawamura & Doi [26] |
B. pumilus Jo2 | Wild type | Henkel AG & Co. KGaA |
B. pumilus Jo2.1 | Jo2 Δyqf D | Wemhoff et al. [13] |
Plasmids | ||
pHP49 | pBC16, Pref, subtilisin BL18, KmR | Henkel AG & Co. KGaA |
pHP59 | pBC16*, Pref, subtilisin BL18, KmR | Henkel AG & Co. KGaA |
pPB49 | pUB110, Pref, subtilisin BL18, KmR | Henkel AG & Co. KGaA |
pHP17 | pUB110*, Pref, subtilisin BL18, KmR | Henkel AG & Co. KGaA |
pVS13 | pHP49, but P apr E1 I | This work |
pVS24 | pHP49, but P apr E1 II | This work |
pVS23 | pHP49, but P apr E1 III | This work |
pVS14 | pHP49, but P apr E1 IV | This work |
pVS19 | pHP49, but P mpr | This work |
pVS20 | pHP49, but P apr E2 | This work |
pHP5-31 | pBC16, Pref, α-amylase, KmR | Henkel AG & Co. KGaA |
pTK5 | pHP5-31, but P mpr | This work |
pTK6 | pHP5-31, but P apr E1 III | This work |
pTK7 | pHP5-31, but P apr E2 | This work |