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Figure 5 | Microbial Cell Factories

Figure 5

From: Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer

Figure 5

Maximum enzyme titer of B. pumilus Jo2.1 cultivations in reference to the current production host B. licheniformis at lab scale: Overexpression of subtilisin BL18 ( B. licheniformis ) vs. subtilisin BL18 ( B. pumilus Jo2.1) and A7-7 α-amylase ( B. pumilus Jo2.1), respectively. Process conditions and specific plasmid configurations are given below the figure. Based on a harshly decreased process robustness expressing the amylase, bars represent the maximum achieved amylase titer of four independent cultivations per strain. Yielded amylase concentrations are scaled to B. licheniformis protease titer at the same scale serving as industrial relevant reference for enzyme production.

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