Fed batch cultivation of B. pumilus Jo2.1/pHP49 at lab scale. Adapted cultivation process based on a down-scaled production process of B. licheniformis. Colony forming Units (CFU) (blue triangle) [L-1], glucose concentration (orange diamond) [g/l], acetate concentration (green square) [g/l] and protease titer (gray square) were measured at line. The pH-value (light blue circle) was monitored online. Yielded enzyme concentrations scaled in correlation to the subtilisin BL18 protease titer of the current production host B. licheniformis in a lab scale cultivation serving as industrially relevant reference in this contribution.