Figure 2

Proteomic fingerprint of cells growing in defined and rich medium. Cells growing at exponential phase in glucose supplemented mineral salt medium (A) and in LB medium at exponential (B) and stationary phases (C) were analyzed by 2D gel electrophoresis. Images of gel sections indicating representative metabolic enzymes are shown: upper glycolysis, FbaB 1; lower glycolysis, GpmA 2, PpsA 3; TCA cycle: GltA 4, SucD 5, Mdh 6; anaplerotic reactions, PckA 7, MaeB 8; by-product metabolism, Acs 9, Pta 10, AckA 11, PoxB 12, AdhE 13; amino acid biosynthesis, MetE 14, IlvE 15, IlvC 16, CysK 17, and amino acid degradation, TnaA 18. Sampling time-points are indicated in Figure 1. The entire gel images representing the proteome of cells grown in defined and rich (LB and TB) media at exponential and stationary phases indicating all identified proteins are given in the Additional file 1: Figures S1-S6.