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Figure 3 | Microbial Cell Factories

Figure 3

From: A new fusion protein platform for quantitatively measuring activity of multiple proteases

Figure 3

Coupled assay of time course cleavage of the designed fusion protein by proteases. A. SDS-PAGE analysis of expressed His6MBP-rpS-eDAL in E. coli cells. U: uninduced; I: induced. The protein is indicated by the arrow. B. Time course cleavage activity of His6MBP-rpS-eDAL from crude extracts using GST fused R3P by coupled assay in the absence or presence of the inhibitor. C. Coupled assay of time course cleavage of two fusion proteins for eDAL and sDAL from crude extracts by the TEVp variant. D. Coupled assay of time course cleavage of the fusion protein from crude extracts by factor Xa. The activity of DAL partner was used as a control and subtracted.

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