Figure 2
From: A new fusion protein platform for quantitatively measuring activity of multiple proteases
Overexpression, purification and activity assay of eDAL with a His6GST followed by the specified protease cleavage site. A. SDS-PAGE analysis of the soluble fusion proteins expressed in E. coli. U: uninduced; I: induced. B. SDS-PAGE analysis of the purified fusion proteins. In the Figure 2 A and 2 B, all the proteins are indicated by arrows. C. DAL activity of the fusion protein in the crude extracts. D. DAL activity of the purified fusion protein.