Table 2 The strains and plasmid used in this study
Strain or plasmids | Description | Source or reference |
|---|---|---|
Strains | Â | Â |
E. coli DH5α | Host for general cloning | TransGen biotech |
E. coli ET-12567 | Donor stain for conjugation between E. coli and S. spinosa | [44] |
S.spinosa ATCC 49460 | Wild strain | ATCC |
S.spinosa 261P | S.spinosa ATCC 49460 harboring pOJ261P | This study |
Plasmid | Â | Â |
pOJ260 | E. coli–Streptomcyes shuttle vector; apr oriT repPUC lacZ | [45] |
pOJ261 | pOJ260 with ermE* promoter | Our lab |
pOJ261P | pOJ260 with ermE*-controlled PntAB | This study |