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Table 2 The strains and plasmid used in this study

From: Genome-scale metabolic network reconstruction of Saccharopolyspora spinosa for Spinosad Production improvement

Strain or plasmids Description Source or reference
Strains   
E. coli DH5α Host for general cloning TransGen biotech
E. coli ET-12567 Donor stain for conjugation between E. coli and S. spinosa [44]
S.spinosa ATCC 49460 Wild strain ATCC
S.spinosa 261P S.spinosa ATCC 49460 harboring pOJ261P This study
Plasmid   
pOJ260 E. coli–Streptomcyes shuttle vector; apr oriT repPUC lacZ [45]
pOJ261 pOJ260 with ermE* promoter Our lab
pOJ261P pOJ260 with ermE*-controlled PntAB This study