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Table 2 Determination of growth (OD 600 ), specific cell growth rate (μ), specific glucose consumption rate (q glu ), specific shikimic acid production rate (q sa ), and specific 3-dehydroshikimic acid production rate (q dhs ) of B. subtilis strains BSSA/pHCMC04/ pDG148-stu and BSSA/pSAAroA/pDGSAAroD

From: Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains

Strains

μ(h-1)

qglu(mmol g-1 h-1)

qsa(mmol g-1 h-1)

qdhs(mmol g-1 h-1)

BSSA/pHCMC04/ pDG148-stu

0.434 ± 0.023

5.199 ± 0.120

0.101 ± 0.011

0.132 ± 0.013

BSSA/pSAAroA/pDGSAAroD

0.332 ± 0.015

4.666 ± 0.132

0.159 ± 0.014

0.048 ± 0.007

  1. Data are from 3 parallel cultivations and standard deviations are provided. B. subtilis strains were cultivaeted 250-mL flasks that contained 50 mL of M9 minimal medium, which was supplemented to 5 g/L glucose. Temperature and the shaking rate of the incubator were held constantly at 37°C and 200 rpm, respectively.
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Microbial Cell Factories

ISSN: 1475-2859

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