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Table 2 Luciferase activity relative to OD 730 in Synechocystis lux prim and lux sec mutants

From: A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803

 

#1

#2

#3

#4

#5

#6

#7

#8

#9

#10

lux prim

2.6 ± 1.4

2.3 ± 0.4

1.8 ± 0.4

2 ± 1.2

2.1 ± 0.7

1.8 ± 0.5

2.2 ± 1.1

1.7 ± 0.6

1.5 ± 0.8

1.5 ± 1

lux sec (1–10)

17.3 ± 3.9

61.8 ± 11.3

16.6 ± 3.6

24.1 ± 6.5

15.3 ± 5.1

23.2 ± 9.6

22.7 ± 9.6

10.4 ± 3.2

10.6 ± 2.9

13.1 ± 3.8

  1. For each strain, the first recombinant and ten independent second recombinants were analyzed. Each suspension was measured in duplicate and the assay was repeated twice with independently grown cultures. Average luminescence values of the mutants are relative to background signal in the untransformed WT Synechocystis strain, which value was set to 1.