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Table 2 Strains used in this study

From: Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway

Name

Genetic background

HIS3 based plasmid

URA3 based plasmid

Reference strain

BdJref

CEN.PK 113-11C

pIYC04 (−−-)

pSP-GM2 (−−-)

Phosphoketolase strains

BdJ01

CEN.PK 113-11C

pBdJ01 (ws2)

pSP-GM2 (−−-)

BdJ02

CEN.PK 113-11C

pBdJ01 (ws2)

pMPa (xpkA, acK)

BdJ03

CEN.PK 113-11C

pBdJ01 (ws2)

pMPp (xpkA, pta)

BdJ04

CB2I20

---

pSP-GM2 (−−-)

BdJ05

CB2I20

---

pMPa (xpkA, acK)

BdJ06

CB2I20

---

pMPp (xpkA, pta)

Ethanol degradation strains

BdJ07

CEN.PK 113-11C

pIYC04 (−−-)

pSPB2N (ws2)

BdJ08

CEN.PK 113-11C

pBdJ02 (ws2, ALD6, ADH2, acs SE L641P)

pSP-GM2 (−−-)

BdJ09

CEN.PK 113-11C

pIYC09 (ALD6, ADH2, acs SE L641P)

pSPB2N (ws2)