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Figure 1 | Microbial Cell Factories

Figure 1

From: Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway

Figure 1

Overview of metabolic engineering strategies. A. Schematic metabolic pathways describing the (over-)expression of the ethanol degradation pathway and the phosphoketolase pathway. The genes of interest are: xpkA (xylulose-5-phosphate phosphoketolase), ack (acetate kinase), pta (phosphotransacetylase), ADH2 (alcohol dehydrogenase 2), ALD6 (acetaldehyde dehydrogenase), acs SE L641P (acetyl-CoA synthetase) and ws2 (wax ester synthase). B. Stoichiometric equations for the conversion of glucose to the product of interest, FAEE, via either the EMP pathway or the combination of PP and PHK pathway. For convenience it was assumed that the acyl-CoA chain was unsaturated and 16 carbons long. Abbreviations: ADP (adenosine diphosphate), NAD+ (nicotinamide adenine dinucleotide), NADPH (nicotinamide adenine dinucleotide phosphate hydrogenase), ATP (adenosine triphosphate), NADH (nicotinamide adenine dinucleotide hydrogenase), NADP+ (nicotinamide adenine dinucleotide phosphate).

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Microbial Cell Factories

ISSN: 1475-2859

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