Table 1 Numbers for ccd B counterselection with or without Dpn I for the targets and cloning protocols used
From: A high-throughput, restriction-free cloning and screening strategy based on ccd B-gene replacement
Gene target | Cloning technology | Dpn I treatment | Positive/Total screened clones | Transformants1 |
|---|---|---|---|---|
bla OXA-48 | RF-cloning | - | 13/14 | n.d. |
bla OXA-245 | RF-cloning | - | 12/14 | n.d. |
bla OXA-48 | EMP-cloning | - | 26/28 | 1550 |
bla OXA-48 | EMP-cloning | + | 27/28 | 800 |
bla OXA-181 | EMP-cloning | - | 13/29 | 32 |
bla OXA-181 | EMP-cloning | + | 11/24 | 24 |