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Table 2 Specific enzyme activities in cell-free extracts measured at 25°C a

From: Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae

Strain XRb XDHc XKd
BP10001 0.08 ± 0.01 0.6 ± 0.1 1.1 ± 0.2
IBB10A02 1.0 ± 0.2 1.2 ± 0.2 1.8 ± 0.3
IBB10B05 1.2 ± 0.1 0.9 ± 0.1 1.9 ± 0.2
  1. aSpecific enzyme activities are presented as μmol/min/mgprotein.
  2. bXylose, 600 mM; NADH, 0.2 mM; 100 mM potassium phosphate buffer, pH 7.0.
  3. cXylitol, 150 mM; NAD+, 2 mM; 100 mM Tris/HCl buffer, pH 9.0.
  4. dXylulose, 4.3 mM; NADH, 0.2 mM; ATP, 5 mM; phosphoenolpyruvate, 1 mM; bovine serum albumine, 0.88 g/L; MgCl2 6H2O, 8.8 mM; KCl, 44 mM; 44 mM HEPES buffer, pH 7.0.