Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae

Table 2 Specific enzyme activities in cell-free extracts measured at 25°C ^a

Strain	XR^b	XDH^c	XK^d
BP10001	0.08 ± 0.01	0.6 ± 0.1	1.1 ± 0.2
IBB10A02	1.0 ± 0.2	1.2 ± 0.2	1.8 ± 0.3
IBB10B05	1.2 ± 0.1	0.9 ± 0.1	1.9 ± 0.2

^aSpecific enzyme activities are presented as μmol/min/mg_protein.
^bXylose, 600 mM; NADH, 0.2 mM; 100 mM potassium phosphate buffer, pH 7.0.
^cXylitol, 150 mM; NAD⁺, 2 mM; 100 mM Tris/HCl buffer, pH 9.0.
^dXylulose, 4.3 mM; NADH, 0.2 mM; ATP, 5 mM; phosphoenolpyruvate, 1 mM; bovine serum albumine, 0.88 g/L; MgCl₂ 6H₂O, 8.8 mM; KCl, 44 mM; 44 mM HEPES buffer, pH 7.0.

ISSN: 1475-2859