Skip to main content

Table 1 Physiological parameters obtained from xylose fermentation performed under anaerobic conditions in sealed flasks at pH 6.5 together with aerobic and anaerobic specific growth rates on xylose and glucose, respectively a

From: Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae

Parameterb

BP10001

IBB10A02

IBB10B05

  

Phase 1

Phase 2

Phase 1

Phase 2

μ xylose AN

n.d.c

0.025 ± 0.002

0.056 ± 0.003

μ xylose AE

n.m.c

0.12 ± 0.01

0.16 ± 0.01

μ glucose AN

0.34d

0.27 ± 0.02

0.26 ± 0.01

q xylose

0.15 ± 0.04

0.50 ± 0.03

0.80 ± 0.04

r ATP

1.1 ± 0.1

2.9 ± 0.2

3.7 ± 0.2

5.7 ± 0.4

6.3 ± 0.2

Y biomass

n.d.c

0.05 ± 0.01

0.07 ± 0.01

Y ethanol

0.35 ± 0.02

0.31 ± 0.02

0.35 ± 0.02

Y xylitol

0.19 ± 0.02

0.03 ± 0.005

0.24 ± 0.01

0.03 ± 0.01

0.20 ± 0.01

Y glycerol

0.06 ± 0.01

0.19 ± 0.02

0.015 ± 0.002

0.11 ± 0.02

0.018 ± 0.001

Y acetate

0.021 ± 0.001

0.07 ± 0.02

0.050 ± 0.002

0.04 ± 0.01

0.03 ± 0.01

Y ribitol

n.d.c

n.d.c

0.012 ± 0.003

n.d.c

0.011 ± 0.002

Y CO2 e

0.35

0.35

0.33

0.37

0.36

C-Balance

0.97

1.00

1.01

0.97

1.04

  1. aConcentrations of 50 g/L and 20 g/L were used for xylose and glucose, respectively; Superscripts AN and AE indicate anaerobic and aerobic cultivation conditions, respectively.
  2. b μ, q xylose, r ATP and Y product are presented as h-1, gxylose/gBM/h, mmolATP/gBM/h and gproduct/gxylose, respectively.
  3. cn.d., not detectable; n.m., not measured.
  4. dData was from Ref. [33].
  5. e Y CO2 was calculated based on stoichiometry of metabolic ethanol and acetate formation.