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Table 1 Physiological parameters obtained from xylose fermentation performed under anaerobic conditions in sealed flasks at pH 6.5 together with aerobic and anaerobic specific growth rates on xylose and glucose, respectively a

From: Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae

Parameterb BP10001 IBB10A02 IBB10B05
   Phase 1 Phase 2 Phase 1 Phase 2
μ xylose AN n.d.c 0.025 ± 0.002 0.056 ± 0.003
μ xylose AE n.m.c 0.12 ± 0.01 0.16 ± 0.01
μ glucose AN 0.34d 0.27 ± 0.02 0.26 ± 0.01
q xylose 0.15 ± 0.04 0.50 ± 0.03 0.80 ± 0.04
r ATP 1.1 ± 0.1 2.9 ± 0.2 3.7 ± 0.2 5.7 ± 0.4 6.3 ± 0.2
Y biomass n.d.c 0.05 ± 0.01 0.07 ± 0.01
Y ethanol 0.35 ± 0.02 0.31 ± 0.02 0.35 ± 0.02
Y xylitol 0.19 ± 0.02 0.03 ± 0.005 0.24 ± 0.01 0.03 ± 0.01 0.20 ± 0.01
Y glycerol 0.06 ± 0.01 0.19 ± 0.02 0.015 ± 0.002 0.11 ± 0.02 0.018 ± 0.001
Y acetate 0.021 ± 0.001 0.07 ± 0.02 0.050 ± 0.002 0.04 ± 0.01 0.03 ± 0.01
Y ribitol n.d.c n.d.c 0.012 ± 0.003 n.d.c 0.011 ± 0.002
Y CO2 e 0.35 0.35 0.33 0.37 0.36
C-Balance 0.97 1.00 1.01 0.97 1.04
  1. aConcentrations of 50 g/L and 20 g/L were used for xylose and glucose, respectively; Superscripts AN and AE indicate anaerobic and aerobic cultivation conditions, respectively.
  2. b μ, q xylose, r ATP and Y product are presented as h-1, gxylose/gBM/h, mmolATP/gBM/h and gproduct/gxylose, respectively.
  3. cn.d., not detectable; n.m., not measured.
  4. dData was from Ref. [33].
  5. e Y CO2 was calculated based on stoichiometry of metabolic ethanol and acetate formation.