Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

Table 2 Comparison of process variables, specific activities and yields for clone 4 under different fed-batch strategies in lab-scale bioreactor after 96 h

Fed-batch strategy	Biomass [OD₆₀₀]	Volumetric lipolytic activity [U mL^-1]	Biomass specific activity [U mL^-1OD₆₀₀ ^-1]	MeOH added [mg]	Final volume [mL]	Activity yield from methanol [U mg^-1 _MeOH]
Glucose feeding	138 ± 4.2	9.8 ± 0.6	0.071	71010	3700	0.51
Low glycerol feeding	140 ± 0.1	12 ± 1.2	0.086	118350	3400	0.34

ISSN: 1475-2859