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Table 2 Comparison of process variables, specific activities and yields for clone 4 under different fed-batch strategies in lab-scale bioreactor after 96 h

From: Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

Fed-batch strategy Biomass [OD600] Volumetric lipolytic activity [U mL-1] Biomass specific activity [U mL-1OD600 -1] MeOH added [mg] Final volume [mL] Activity yield from methanol [U mg-1 MeOH]
Glucose feeding 138 ± 4.2 9.8 ± 0.6 0.071 71010 3700 0.51
Low glycerol feeding 140 ± 0.1 12 ± 1.2 0.086 118350 3400 0.34