Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

Table 1 Comparison of process variables, specific activities and yields for clone 4 under different fed-batch strategies in microbioreactor after 96 h

Fed-batch strategy	Biomass [OD₆₀₀]	Volumetric lipolytic activity [U mL^-1]	Biomass specific activity [U mL^-1OD₆₀₀ ^-1]	MeOH added [mg]	Final volume [mL]	Activity yield from methanol [U mg^-1 _MeOH]
Glucose feeding	255	10.3	0.040	19	0.794	0.43
Low glycerol feeding	169	10.3	0.061	28.2	0.898	0.33
High glycerol feeding	222	16	0.072	56.4	1.026	0.29

ISSN: 1475-2859