Estimated response | UoM | Average ± one std. dev |
|---|
10 L scale | 10,000 L |
|---|
| Â | Â | (n=9) | Scale (n=7) |
|---|
Y
O2
| g DCW/mol O2 | 19.7 ± 1.4 | 17.5 ± 1.1 |
Y
p
| mg Pr-1/mol Gluc | 0.63 ± 0.04 | 0.75 ± 0.05 |
q
p
| mg Pr-1/g Gluc/hr | 0.043 ± 0.003 | 0.050 ± 0.003 |
Y
s
| g DCW/g Gluc | 0.39 ± 0.02 | 0.39 ± 0.01 |
- The biomass yields based on oxygen, Y
O2, are defined as amount of biomass (grams of dry cell weight (DCW)) produced per mol of molecular oxygen (O2) consumed by cells (g DCW / mol O2). The biomass yields based on carbon substrate, Y
s, are defined as amount of biomass (grams of DCW) produced per gram of glucose fed (g DCW / g Gluc). The product yields based on carbon substrate, Y
p, are defined as amount of secreted product (milligrams of protein Pr-1) per mol of glucose fed (mg Pr-1 / mol Gluc). The specific rate of therapeutic protein production, q
p, is expressed as milligrams of product Pr-1 per gram of glucose fed and per hour of elapsed fermentation time (mg Pr-1 / g Gluc/hr). The average and one standard deviation of every estimated response indicated above come from 7 batches at 10,000Â L scale, or from 9 batches at 10Â L scale. UoM: unit of measurement.
