Figure 5
Performance differences between two scales. Seven batches, Batch 4–10, were completed at DO 25% and doubled back pressure at the 10,000 L scale bioreactor. Nine runs, run 9–17, were completed in parallel with seven 10,000 L scale batches running at DO 12.5% and doubled back pressure at the 10 L scale bioreactor. (A) WCW profile comparison between two scales. (B) Product titer measured at the culture supernatant profile comparison between two scales. (C) Culture apparent viscosity measured at shear rate of 750 s−1 profile comparison between scales. Only run 12–17 were measured the apparent viscosity at the 10 L scale. Error bars represents “mean +/− one standard deviation”.