Figure 4

Overall consistent fermentation performance between two scales after fine tuning adjustments. Batch 3 was scaled-up and run at DO 25% and doubled back pressure at the 10,000 L scale bioreactor. Six more batches, Batch 4–10, were completed at the same conditions at the 10,000 L scale. Nine more runs, run 9–17, were completed in parallel with seven 10,000 L scale batches running at DO 12.5% and doubled back pressure at the 10 L scale bioreactor. (A) Online process parameters control for 10,000 L scale Batch 3. (B) Mass transfer coefficient comparison between two scales with Batch 3–5 at the 10,000 L scale and Run 9–11 at the 10 L scale. (C) Agitator power draw and agitation profiles for Batch 3–5 at 10,000 L scale. There was an interrupted gas flow at ~ EFT 37 hr at Batch 4, causing a spike in agitator power draw. (D) OUR comparison between two scales with 7 batches at 10,000 L scale and 9 runs at the 10 L scale. (E) Broth titer comparison between two scales with 7 batches at 10,000 L scale and 9 runs at 10 L scale. (F) Dry cell weight (DCW) comparison between two scales with 7 batches at 10,000 L scale and 9 runs at 10 L scale. Error bars represents “mean +/− one standard deviation”.