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Figure 2 | Microbial Cell Factories

Figure 2

From: Exometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch biopharmaceutical process

Figure 2

Up-scaling the fermentation process at the large (10,000 L) scale. Batch 1 and 2 were scaled-up and run at DO 12.5% at the large (10,000 L) scale bioreactor. Run 5 and 7 were consistency runs at DO 12.5% at the 10 L scale bioreactor. (A and D) Online control of process parameters, DO, air sparge rate and oxygen sparge rate, for Batch 1 and 2, respectively. Arrows, “a”, “b” and “c”, indicate the bolus addition of salt feed solution. Arrows, “d”, indicates the bolus addition of water. Additional salt solution was gradually added until the EOR, to hold the pure oxygen sparge supply below 90% of the maximum capacity. (B) OUR comparison between two scales with Batch 1 and Run 5 and 7. (C) WCW and broth titer comparison between Batch 1 and 2 at the 10,000 L scale.

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Microbial Cell Factories

ISSN: 1475-2859

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