Figure 4
From: A system of vectors for Bacillus subtilis spore surface display
Western blotting analysis of expression of the cgeA-cagA fusion gene. Spore coat proteins were extracted and analysed by western blotting with anti-CgeA (panel A) or anti-CagA (panel B) antibodies. Spore coat proteins from spores of the 168 (lane 1), the BAV08 (inactivation of cgeA) (lane 2), the BAV05 (fusion CgeA-CagA) (lane 3) and the BAV07 (fusion CgeA-linker-CagA) (lane 4) strains. Each lane of panel A and B was loaded with 20 μg of total proteins.