Figure 5
Activity assays of the purified MB109. (A) Smad1-depend luciferase reporter assay in mouse myoblast C2C12 cells. Black circles are E. coli-derived MB109. Gray circles are CHO-derived hBMP-9. Black squares are E. Coli-derived hBMP-2. S.E.M. are shown, n = 3. (B) Binding affinities of MB109 to Type I and Type II receptors as determined by surface plasma resonance. ECD-Fc, extracellular domain with C-terminal human IgG1 tag. ND, non-detectable (no signal above the background detection was detected in all tested protein concentration ranging from 20 to 0.3 nM).