Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium

Table 1 E. coli SA engineered overproducing strains, growth conditions and SA yield

Strain/derivative	Relevant characteristics	Culture conditions	SA [g/L]	Yield (mol SA/mol glucose)	Reference
SP1.1/pKD12.138	serA::aroB ΔaroL ΔaroK pSU18aroF ^fbrP^tac aroE serA tktA	1-L fed-batch cultures, mineral broth with 25 g/L of glucose and 15 g/L of YE	52	0.18	[8]
SP1.1pts/pSC6.090B	PTS⁻ serA::aroB ΔaroL ΔaroK PTS⁻ P^tac glf glk aroF ^fbr tktA P^tac aroE serA, glf ¹ glk ²	10-L fed-batch reactors, mineral broth with 25 g/L of glucose and 15 g/L of YE	71	0.27	[8]
W3110.shik1	ΔaroL aroG ^fbr aroF ^fbr tnaA, and plasmid overexpressed aroK	Chemostat cultures using mineral broth under glucose limiting conditions	NR	~0.08	[9]
PB12.SA22 (JM101 derivative)	PTS⁻ ΔaroL ΔaroK pJLBaroG ^fbr tktA pTOPO aroB aroE	0.5-L batch reactors, mineral broth with 25 g/L of glucose and 15 g/L of YE	7	0.29	[10]
DHPYAAS-T7(DH5α- derivative)	ΔptsHIcrr ΔaroL Δarok ΔydiB knock-in of T7-RNA-pol gene, pAOC-TGEFB aroE aroB, site-specific mutagenesis glk tktA aroF ^fbr	Fed-batch fermentation, modified M9 medium, with 25 g/L of glycerol and 25 g/L of YE	1.85	NR	[11]
AR36 (JM101 derivative)	PB12 lacI ⁻ aroK ⁻ aroL ⁻ pykF ⁻ Trc/aroB ⁺ tktA ⁺ aroG ^fbr+ aroE ⁺ aroD ⁺ zwf ⁺	Batch fermentation, mineral broth with 100 g/L of glucose and 30 g/L of YE	43	0.42	[12]
E. coli SA116	Chromosomally evolved and cofactor metabolic engineered strain	Mineral broth with 10 g/L of glucose, 1 g/L of peptone, 1.24 g/L of proline	3.12	0.33	[13]

¹Glucose facilitator (glf) and ²Glucokinase (glk) from Zymomonas mobilis; NR, Non-reported.

ISSN: 1475-2859