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Figure 7 | Microbial Cell Factories

Figure 7

From: Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa

Figure 7

SDS-PAGE gel analysis and Western blot validation of samples from the four time phases of S. spinosa. (A) SDS-PAGE gel analysis. M: pre-stained protein marker; Lanes 1 to 4: protein samples from T1, T2, T3, and T4 of S. spinosa cells, respectively; Arrows 1 to 7: gel bands selected for 1D-LC-MS/MS identification. (B) A table list of proteins identified by 1D-LC-MS/MS. The protein with the highest score for a gel band was considered the corresponding protein of the said gel band. (C) Western blot validation of the abundance of CNDP, GS, and MHSM expression in S. spinosa. M: pre-stained protein marker; Lanes 1 to 4: protein samples from T1, T2, T3, and T4 of S. spinosa cells, respectively; Lanes 5 to 7: positive controls of CNDP, GS, and MHSM [the purified heterologous expression proteins].

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Microbial Cell Factories

ISSN: 1475-2859

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