Figure 1

Growth parameters of the S. spinosa CCTCC M206084 fermentation culture. The growth curve (red line) was drawn according to the OD₆₀₀ values, whereas the spinosad yield curve (green line) and glucose consumption curve (brown line) were drawn according to the analysis by HPLC, and the pH value (blue line) were taken to monitor growth every 24 h. Samples for proteomic analysis were collected at 2, 4, 8, and 12 d post-inoculation from fermentation cultures, as T1, T2, T3, and T4 phases, respectively. The logarithmic phase was from 0 d to T2, the stationary phase was from T2 to T3, whereas the decline phase was from T3 to T4.