The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage

Shafiei, Rasoul; Zarmehrkhorshid, Raziyeh; Bentaib, Azeddine; Babanezhad, Manoochehr; Leprince, Pierre; Delvigne, Frank; Thonart, Philippe

doi:10.1186/1475-2859-13-26

Microbial Cell Factories

Table 2 Some cellular proteins modifications of freeze-dried A. senegalensis stored at 35°C for 12 months

From: The role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage

Master no.	Accession no.	Proteins	Modifications	MW (kD)	pI¹	Modified amino acid and site of carbonylation	Score/number of peptides/Percentage of coverage
*Shift of pI*
1254	gi\|258542052	LSU ribosomal protein L17P	Shift of pI to about 4.5-5	15.5	10.8	---	254/6/36
1456	gi\|258542952	LSU ribosomal protein L21P	Shift of’ pI to about 4.5-5	13.1	11.4	---	196/4/31
1697	gi\|258542032	LSU ribosomal protein L22P	Shift of’ pI to about 4.5-5	15	11.5	---	229/4/30.9
*Carbonylated proteins*
1581	gi\|258542025	Translation elongation factor Tu	Threonine carbonylation	43.3	5.1	Thr: 111	83/5/50
934	gi\|258542743	Translation elongation factor G	Proline carbonylation	76.9	5.2	Pro: 651, 681, 683	85/13/20
914	gi\|258542743	Translation elongation factor G	Proline carbonylation	76.9	5.2	Pro: 681 and 683	122/13/24
1313	gi\|258541164	SSU ribosomal protein S1	Proline carbonylation	63	5.2	Pro: 262	80/7/15
773	gi\|258542981	Pyruvate phosphate dikinase	Proline carbonylation Threonine carbonylation Arginine carbonylation	96.7	5.5	Pro: 758, 623, 670	170/21/17
773	gi\|258542981	Pyruvate phosphate dikinase		96.7	5.5	Thr: 290 Arg:579	170/21/17
1552	gi\|271502383	Putative lipoprotein	Threonine carbonylation	6.6	9.86	Thr: 9	79/5/96
1368	gi\|251798976	PBS lyase	Threonine carbonylation Lysine carbonylation	42.1	6.72	Thr: 373	86/8/24
1368	gi\|251798976	PBS lyase	Threonine carbonylation Lysine carbonylation	42.1	6.72	Lys: 86, 236	86/8/24
1392	gi\|258541222	ATP synthase F1 beta subunit	Proline carbonylation Arginine carbonylation	52.6	4.6	Pro: 238, 359, 364	125/15/39
1392	gi\|258541222	ATP synthase F1 beta subunit	Proline carbonylation Arginine carbonylation	52.6	4.6	Arg: 68, 289	125/15/39
1402	gi\|258541220	ATP synthase F1 alpha subunit	Proline carbonylation	55.4	5.4	Pro: 5	79/9/20
1540	gi\|258542167	Acetyl-CoA hydrolase	Proline carbonylation	54.9	6.2	Pro: 460, 464	88/10/24

¹The theoretical iso-electric point.

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ISSN: 1475-2859

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