Figure 5

2D-DiGE of A. senegalensis preserved at −21 (left), 4 (middle) and 35°C (right). The proteins were pre-stained with Cy3 before separation. As it is evident, slight and moderate streaking and trains of spots were formed in the proteome pattern of cells kept at 4 and 35°C. The density of high molecular weight proteins at the basic side of the gel decreased at 35°C. The selected sections (white and black rectangles) show clearly the differences between the patterns of proteins.