Figure 10

Identification of carbonylated proteins of freeze-dried A. senegalensis stored at 35°C after separation by 2D-electrophoresis. A preparative gel and a western blot gel containing the same sample were run in parallel. Anti-DNP antibody staining was realized on the WB to localizethe carbonylated proteins spots that were then reported on the preparative gel image, as indicated by the blue spots. Eighty-eight proteins which underwent carbonylation were then picked from the preparative gel and analyzed by mass spectroscopy. Thirteen different proteins were identified as indicated on the gel image, of which 10 are carbonylated proteins with modifications in Arginine, Lysine, Proline and Threonine as listed on Table 2.