Figure 8
From: Effect of metal catalyzed oxidation in recombinant viral protein assemblies
Assembly efficiency of VP6 exposed to MCO for 1 h (150 µM of FeCl 2 ). 1 mg of VP6U at 0.4 mg/mL was subjected to assembly conditions. A) SEC of samples of VP6 after incubation at the assembly conditions. For comparison, a chromatogram of VP6U is also shown. B) Assembly efficiencies calculated from chromatograms in A. Assembly efficiencies were calculated as the area under the curve of peak 1 of each chromatogram divided by the total area below the curve at KD lower or equal to 0.8. Experiments were performed in duplicate, difference between duplicates is represented by error bars.